development of pcr method for diagnosing of honey bee american foulbrood disease
نویسندگان
چکیده
american foulbrood (afb) disease is caused by the sporeforming bacterium paenibacillus larvae larvae. traditional diagnosis is based on culture technique is time and laboratory work consuming. in this study with standard strain, pcr was developed by specific primers and pcr products were electrophoresed on 0.8 % agarose gel. the pcr primers were selected on the basis of the 16s rrna gene and amplify a 700-bp amplicon. detection limits were determined for suspensions of bacteria and spores and also honey and larvae experimentally contaminated. the lowest number of bacteria and spores that were able to detect were respectively 28, 33, 330 and 243 cfu /ml. this pcr technique can be used to identification of the presence of paenibacillus larvae larvae spores in honey samples, brood samples or on the colonies that grow on medium.
منابع مشابه
Development of PCR method for diagnosing of honey bee American Foulbrood disease
American foulbrood (AFB) disease is caused by the sporeforming bacterium Paenibacillus larvae larvae. Traditional diagnosis is based on culture technique is time and laboratory work consuming. In this study with standard strain, PCR was developed by specific primers and PCR products were electrophoresed on 0.8 % agarose gel. The PCR primers were selected on the basis of the 16S rRNA gene and am...
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American foulbrood disease (AFB), caused by the bacterium Paenibacillus larvae, is a disease of the European honey bee (Apis mellifera) and other honey bee species (Apis sp.). It is distributed world wide. Spores of the bacterium can infest larvae of the bees, ultimately leading to the death of the larva or pupa. Although often spores are present in bee colonies, it only sporadically leads to s...
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american foulbrood disease (afb) is one of the most important bacterial diseases of honey bee can be caused severe losses in apiaries. afb caused by paenibacillus larvae a gram positive and spore-forming bacterium. this study was taken placed in 15 area of east azerbaijan province of iran between 2006-2007 years. in this study, 500 bee hives were randomly selected from 100 apiaries. standard ba...
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عنوان ژورنال:
archives of razi instituteناشر: razi vaccine & serum research institute (rvsri)
ISSN 0365-3439
دوره 67
شماره 1 2016
کلمات کلیدی
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